S. Desantis, M. Labate, F. Cirillo, G. M. Labate, P. Maiorano and G. D'Onghia

Laboratory of Comparative Anatomy and Cytology, Department of Zoology
University of Bari, Via E. Orabona 4, 70125 Bari, Italy

Source - Journal of Northwest Atlantic Fishery Science, Volume 31: 205-212
ISSN-0250-6408

Desantis, S., Labate, M., Cirillo, F., Labate, G.M., Maiorano, P., and D'Onghia, G. 2003. Testicular Activity and Sperm Glycoproteins in Giant Red Shrimp (Aristaeomorpha foliacea). J. Northw. Atl. Fish. Sci., 31: 333-338. https://doi.org/10.2960/J.v31.a15

Abstract 

The reproduction of male giant red shrimp Aristaeomorpha foliacea, collected from the late winter to the summer in the north-western Ionian Sea (Mediterranean Sea), was investigated using histological and histochemistry methods. Seasonal changes in the spermiogenesis and the glycoprotein pattern were found and sperm glycoproteins matured as gametes moved from the testis to the terminal ampulla. In serial sections stained with hematoxylin and eosin the testicular activity appeared to be discontinuous. In late winter the testes had no meiotic activity and the seminiferous epithelium consisted of interkinetic spermatogonia and spermatozoa. In spring, spermiogenetic activity was high and the seminiferous epithelium mainly consisted of spermatocytes and spermatozoa while in summer, the testes were again inactive since both spermatocytes and spermatozoa were lacking. The use of twelve different lectins indicated that the intratesticular spermatozoa from late winter to summer contain surface binding sites for SNA, MAA, Con A and KOH-sialidase (si)-WGA. In March and July they also exhibited nuclear and cytoplasmic reactivity for SNA and Con A. In the hemispermatophore the spermatozoa displayed a more complex lectin-binding pattern because they also reacted with PNA, DBA, HPA, GSA II. The staining with DBA, KOH-si-DBA, and GSA II showed differences between the spermatozoa from late winter-spring hemispermatophores and summer hemispermatophores: the former showed a nuclear affinity whereas the latter displayed surface and/or cytoplasm staining. No reaction was observed with SBA, GSA I-B4, UEA I, and LTA.

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Language - English
Publisher - Northwest Atlantic Fisheries Organization (NAFO), Dartmouth, N.S., Canada
Publication Date - 2003
Publication Type - Journal Article
Descriptors - histology, Mediterranean, reproduction, shrimp

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